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Visualization of human sperm chromosomes by using mouse oocytes cryothawed after enucleation.

Araki Y, Yoshizawa M, Araki Y

Science of Plant and Animal Production, United Graduated School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan. yaaraki@nifty.com

OBJECTIVE: To evaluate whether previously enucleated mouse oocytes that are vitrified are usable for the analysis of human sperm chromosomes. DESIGN: Prospective, comparative laboratory study. SETTING: Animal breeding and reproduction department in a rural Japan university. PATIENT(S): Sperm samples were obtained from a fertile donor. INTERVENTION(S): Human sperm were injected into previously enucleated-vitrified mouse oocytes. MAIN OUTCOME MEASURE(S): The rates of appearance of the human sperm chromosomes that were injected into previously enucleated-vitrified mouse oocytes. RESULT(S): The rates of appearance of human sperm chromosomes were 71%-72% in enucleated-vitrified mouse oocytes. CONCLUSION(S): The use of enucleated-vitrified mouse oocytes allowed visualization of human sperm chromosomes.

Published 2 August 2006 in Fertil Steril, 86(2): 348-51.
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