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Normal birth after transfer of cryopreserved human embryos generated by microinjection of cryopreserved testicular spermatozoa into cryopreserved human oocytes.

Levi Setti PE, Albani E, Novara PV, Cesana A, Bianchi S, Negri L

Operative Unit Reproductive Medicine, Istituto Clinico Humanitas, Rozzano (Milan), Italy. paolo.levi_setti@humanitas.it

OBJECTIVE: To report the first birth after transfer of cryopreserved embryos generated by intracytoplasmic sperm injection of cryopreserved testicular spermatozoa into cryopreserved human oocytes. DESIGN: Case report. SETTING: Tertiary center for reproductive technology. PATIENT(S): A 36-year-old woman with primary infertility of 3 years' duration and a 37-year-old man with congenital bilateral absence of the vas deferens. INTERVENTION(S): Cryopreservation of human embryos after oocytes and sperm thawing. MAIN OUTCOME MEASURE(S): Live birth. RESULT(S): A healthy, normal female infant with a birth weight of 2,950 g was born by cesarean section at 38 weeks' gestation, with normal 6-month follow-up. CONCLUSION(S): Embryo cryopreservation can lead to successful results, even with the use of cryopreserved gametes.

Published 11 April 2005 in Fertil Steril, 83(4): 1041.
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